master cycler nexus gradient thermal cycler (Eppendorf AG)

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Eppendorf AG master cycler nexus gradient thermal cycler
Master Cycler Nexus Gradient Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 199205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/master+cycler+nexus+gradient+thermal+cycler/Eppendorf/pm41896298-227-11-17
Average 99 stars, based on 199205 article reviews

master cycler nexus gradient thermal cycler - by Bioz Stars, 2026-09

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Article Title: Immunological responses and gut microbial shifts in Phthorimaea absoluta exposed to Metarhizium anisopliae isolates under different temperature regimes
Article Snippet: The experiments were conducted using a Master Cycler Nexus gradient thermal cycler (Eppendorf, Germany), where the reactions were set up in a total reaction volume of 50 μL.

Article Title: Gut microbiota shift in layer pullets fed on black soldier fly larvae-based feeds towards enhancing healthy gut microbial community
Article Snippet: The reactions were set up in a total reaction volume of 50 μL and run in a Master cycler Nexus gradient thermal cycler (Eppendorf, Germany), under the following conditions: initial denaturation for 2 min at 95 °C, followed by 35 cycles of denaturation for 30 s at 95 °C, annealing for 40 s at 55 °C, extension for 1 min at 72 °C, and a final extension step of 10 min at 72 °C.

Article Title: Association of Endothelial Nitric Oxide Synthase Gene Polymorphisms with Coronary Artery Disease in North Indian Punjabi Population.
Article Snippet: Nitric oxide (NO) a potent vasodilator synthesized by endothelial cells has anti-atherosclerotic properties and maintains vascular tone.. It has been documented that its reduced bioavailability in vascular endothelium plays an important role in the development and progression of coronary artery disease (CAD).. Therefore, we aimed to investigate the association of − 786 T > C and 894 G > T polymorphisms of eNOS with CAD.

Article Title: Gut microbiota shift in layer pullets fed on black soldier fly larvae-based feeds towards enhancing healthy gut microbial community.
Article Snippet: The reactions were set up in a total reaction volume of 50 μL and run in a Master cycler Nexus gradient thermal cycler (Eppendorf, Germany), under the following conditions: initial denaturation for 2 min at 95 °C, followed by 35 cycles of denaturation for 30 s at 95 °C, annealing for 40 s at 55 °C, extension for 1 min at 72 °C, and a final extension step of 10 min at 72 °C.

Article Title: Mitogenomic profiling and gut microbial analysis of the newly identified polystyrene-consuming lesser mealworm in Kenya
Article Snippet: The reactions were run in a Master cycler Nexus gradient thermal cycler (Eppendorf, Germany) under the following conditions: initial denaturation for 2 min at 95 °C, followed by 40 cycles of denaturation for 30 s at 95 °C, annealing for 45 s at 55 °C, extension for 1 min at 72 °C, and a final extension step of 10 min at 72 °C.

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Article Title: Diversity and cellulolytic activity of culturable bacteria isolated from the gut of higher termites (Odontotermes sp.) in eastern Thailand
Article Snippet: Polymerase chain reaction (PCR) amplification of the 16S rRNA genes was carried out using an OnePCR reaction mixture (Bio-Helix, Taiwan) with a universal 27F primer (5-AGAGTTTGATCMTGGCTCAG-3) and a universal 1492R primer (5-TACGGYTACCTTGTTACG ACTT-3) for 35 amplification cycles. .. The amplification parameters were conducted according to Boontanom and Chantarasiri (2020) in a Master cycler Nexus Gradient thermal cycler (Eppendorf, Germany), which involved a preheating step at 94C for 4 minutes, a denaturation step at 94C for 40 seconds, an annealing step at 55C for 1 minute, an extension step at 72C for 1 minute 10 seconds, and a final extension step at 72C for 10 minutes. .. The restriction fragment length polymorphism (RFLP) analysis of the PCR products was performed by two restriction enzymes of MspI and AluI (New England Biolabs, UK) in a CutSmart buffer (New England Biolabs, UK) following the previous protocol described by Chantarasiri (2020).

Article Title: Temperature dependent immunological responses of Spoladea recurvalis exposed to entomopathogenic fungi
Article Snippet: The library was prepared using a 50 μl mix containing; PCR Water (20.7 μl) (New England Biolabs, US), MyTaq buffer (8 μl) (Meridian Biosciences, UK), MyTaq enzyme (0.5 μl) (Meridian Biosciences, UK), 25mM.MgCl 2 (0.8 μl) (New England Biolabs, US), 16 S Barcode (10 μl) (Oxford Nanopore Technologies) and DNA template (10 μl). .. Amplification was done using Master Cycler Nexus gradient thermal cycler (Eppendorf, Germany) by running 1 cycle for 2 min at 95 °C for initial denaturation, 40 cycles for 30 s at 95 °C for denaturation, 40 cycles for 45 s at 55 °C for annealing, 40 cycles for 1 min at 72 °C for extension, 1 cycle for 10 min at 72 °C for final extension and then was held at 10 °C. .. Amplicons were then purified using AMPure beads following the ONT protocol and checked for quality and quantity using Nanodrop 2000 Spectrophotometer, sampled and pooled to a 1.5 ml Eppendorf DNA LoBind tube and 1 μl of RAP adapters (Oxford Nanopore Technologies, UK) added.

Article Title: Temperature dependent immunological responses of Spoladea recurvalis exposed to entomopathogenic fungi.
Article Snippet: The library was prepared using a 50 μl mix containing; PCR Water (20.7 μl) (New England Biolabs, US), MyTaq buffer (8 μl) (Meridian Biosciences, UK), MyTaq enzyme (0.5 μl) (Meridian Biosciences, UK), 25mM.MgCl2 (0.8 μl) (New England Biolabs, US), 16S Barcode (10 μl) (Oxford Nanopore Technologies) and DNA template (10 μl). .. AR TIC LE IN PR ES S Amplification was done using Master Cycler Nexus gradient thermal cycler (Eppendorf, Germany) by running 1 cycle for 2 minutes at 95°C for initial denaturation, 40 cycles for 30 seconds at 95°C for denaturation, 40 cycles for 45 seconds at 55°C for annealing, 40 cycles for 1 minute at 72°C for extension, 1 cycle for 10 minutes at 72°C for final extension and then was held at 10°C. .. Amplicons were then purified using AMPure beads following the ONT protocol and checked for quality and quantity using Nanodrop 2000 Spectrophotometer, sampled and pooled to a 1.5 ml Eppendorf DNA LoBind tube and 1 μl of RAP adapters (Oxford Nanopore Technologies, UK) added.

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